EVALUATION OF THE ANTI-DYSLIPIDEMIC PROPERTY OFn-HEXANE LEAF EXTRACT OF FLAMING GLORY FLOWER (Clerodendrum splendens) IN MALE ALBINO RATS.

Abstract

Glory flower (C.splendens) is a plant that has been used for the treatment of various ailments in traditional medicine. The current study is carried out to establish and ascertain the level of the medicinal potentials of n-hexane leaf extract of Clerodendrum splendens. The quantitative determination of the fatty acid components of this extract was carried out using GC-MS Analysis. Modulation of dyslipidemia by n-hexane extract of Clerodendrum splendens leaf in selected tissues of male albino rats; the effect of the n-hexane extract of Clerodendrum splendens leafon liver and kidney function biomarkers as well as antioxidant profiles were determined to establish its role in the management dyslipidemic disorder in selected tissues of male albino rats. Fatty acids identified in leaf sample accounted for the ratio of -6:-3 polyunsaturated fatty acids present as approximately 2:1 and Linoleic acid, an omega-6 fatty acid as having the highest percentage (41.65%) with Capric acid having the lowest percentage (0.0021%). Other fatty acids present are Palmitic (29.33%), Linolenic (16.96%), Oleic (5.03%) andStearic (3.65%) acids in significant amounts. Lipid profile assay showed marked decrease of total cholesterol, TAG, VLDL and LDL at the end of the experiment in all Clerodendrum splendens administered groups of serum and different tissues compared to the control and reference groups while groups of rats administered with the sample shows higher levels of HDL-C when compared to the untreated groups. Liver function markers reveal increased activity of AST (36.37 U/I) when compared to ALP (34.28 U/I), ALT (18.27 U/I) and GGT (12.61 U/I) in that order but all these enzymes have reduced activity in all the administered groups with C.splendensextract. Reductions were (23.98 U/I, 18.91 U/I, 11.52 U/I & 7.22 U/I respectively), most especially at the 400mg/kg BW/0.4ml w/v Olive oil concentration. Kidney function tests reveal significant decrease in creatinine and urea nitrogen activities in the entire treated groups compared to the control and reference groups. There was a significant decrease in kidney urea (6.44 mg/dl) and creatinine levels (0.72 mg/dl) in all the groups for 21days period of administration. Antioxidant activity show significant increase in activity in SOD (superoxide dismutase) at the 100mg/kg BW/0.4ml w/v (155.98 μmol/min/g tissue), (161.54 μmol/min/g tissue) at the L2 (200mg/kg BW/0.4ml w/v) and (166.12 μmol/min/g tissue) at the 400mg/kg BW/0.4ml w/v Olive oil concentration compared to the Control and Reference groups (149.92, 152.86 μmol/min/g tissue) respectively. GSH (reduced glutathione) at L1 (55.98 mg/tissue), L2 (62.67 mg/tissue) and L3 (68.22 mg/ g tissue), CAT (catalase) at L1 (99.99 μmol/min/g tissue), L2 (104.33 μmol/min/g tissue) and L3 (111.19 μmol/min/g tissue), which have increased activity compared to the control and reference groups (90.46 μmol/min/g tissue and 97.26 μmol/min/g tissue) respectively. GST (Glutathione s-transferase) at L1 (9.15 μmol/min/g), L2 (12.99 μmol/min/g) and L3 (13.08 μmol/min/g) in all groups administered with C. splendens extract compared to the control and reference group (5.43 μmol/min/g and 8.12 μmol/min/g). The findings suggest that Clerodendrum splendens could be used as a food supplement in the management of ailments and oxidative stress related diseases.